The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 13, 2011
Filed:
Jul. 28, 2006
Rafael Rodriguez Puertas, Leioa-Vizcaya, ES;
Gabriel Barreda Gomez, Leioa-Vizcaya, ES;
Maria Teresa Giralt Rue, Leioa-Vizcaya, ES;
Begona Fernandez Pastor, Castelldefels-Barcelona, ES;
Rafael Rodriguez Puertas, Leioa-Vizcaya, ES;
Gabriel Barreda Gomez, Leioa-Vizcaya, ES;
Maria Teresa Giralt Rue, Leioa-Vizcaya, ES;
Begona Fernandez Pastor, Castelldefels-Barcelona, ES;
Universidad Del Pais Vasco, Leioa-Vizcaya, ES;
Abstract
The invention relates to a method for quantifying G protein-coupled receptor (GPCR)-G protein binding by means of using a cell membrane array, which comprises (i) putting an unlabeled candidate compound in contact with a cell membrane array in the presence of labeled GTP or of a labeled, non-hydrolyzable analog thereof, in conditions allowing the interaction between said compound and said GPCR present in said cell membranes, and between said labeled GTP or analog thereof and said G protein present in said membranes; (ii) washing; and (iii) quantifying the signal obtained due to the binding of the labeled GTP (or analog) to said G protein. It is applicable in the analysis of the interaction between compounds and cell membrane receptor proteins and of the intracellular signaling mechanisms triggering this interaction mechanism mediated by said compounds.