The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 06, 2011
Filed:
Oct. 04, 2006
Takahiro Yano, Hiratsuka, JP;
Tetsuji Nagasawa, Ikeda, JP;
Akiko Temma, Suita, JP;
Naho Suzuki, Itami, JP;
Kazue Miyaji, Ikeda, JP;
Takahiro Yano, Hiratsuka, JP;
Tetsuji Nagasawa, Ikeda, JP;
Akiko Temma, Suita, JP;
Naho Suzuki, Itami, JP;
Kazue Miyaji, Ikeda, JP;
GenomIdea, Inc., Ibaraki-shi, Osaka, JP;
Abstract
A novel cell suited for mass production of Hemagglutinating Virus of Japan (HVJ), a method for obtaining the cell and use of the cell are disclosed. The human cell is originated from a transformed human kidney cell line, the doubling time thereof in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, the cell has a freeze-recovery property, the maximum density of viable cells in suspension culture is not less than 10cells/mL, and HVJ can grow in the cell. The method for obtaining the human cell comprises the steps of suspension-culturing a human transformed kidney cell line in a serum-free medium, and cloning the grown cells; and selecting, from the cloned cells, a cell whose doubling time in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, which has a freeze-recovery property, whose maximum density of viable cells in suspension culture is not less than 10cells/mL, in which HVJ can grow.