The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 16, 2011

Filed:

Jul. 27, 2006
Applicants:

Kazutaka Tsuruhami, Gifu, JP;

Shigeharu Mori, Gifu, JP;

Yoshinao Koide, Gifu, JP;

Inventors:

Kazutaka Tsuruhami, Gifu, JP;

Shigeharu Mori, Gifu, JP;

Yoshinao Koide, Gifu, JP;

Assignee:

Amano Enzyme Inc., Gifu, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/24 (2006.01); C12N 1/00 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

A novel diglycosidase produced by a microorganism belonging to the genus, having the following physicochemical properties: (1) action and substrate specificity: it acts on a disaccharide glycoside, releasing the disaccharide sugar and the aglycone thereof; (2) optimum pH: around 4.5; (3) pH stability: it is stable at pH 4.0 to 8.0 under the processing condition of 37° C. for 30 minutes, and retains its 80% or more of the activity even after processing at pH 4.0 or lower; (4) optimum temperature: around 60° C. in a sodium acetate-acetic acid buffer solution (pH 5.5); (5) thermal stability: it is stable at 50° C. or lower in a sodium acetate-acetic acid buffer solution (pH 5.5) and retains 45% of the activity even after processing at 60° C. for 40 minutes; (6) molecular weight: 40,000±5,000 Da based on SDS-PAGE measurement; and (7) isoelectric point (pI): about 4.3.


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