The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 09, 2011
Filed:
Feb. 17, 2001
Florian Kern, Berlin, DE;
Hans-dieter Volk, Berlin, DE;
Petra Reinke, Berlin, DE;
Nicole Faulhaber, Rheurdt, DE;
Ingolf-pascal Surel, Berlin, DE;
Elham Khatamzas, Berlin, DE;
Florian Kern, Berlin, DE;
Hans-Dieter Volk, Berlin, DE;
Petra Reinke, Berlin, DE;
Nicole Faulhaber, Rheurdt, DE;
Ingolf-Pascal Surel, Berlin, DE;
Elham Khatamzas, Berlin, DE;
Other;
Abstract
The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.