The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 12, 2011
Filed:
Sep. 02, 2005
Michael Seul, Fanwood, NJ (US);
Nataliya Korzheva, Somerville, NJ (US);
Jiacheng Yang, Hillsborough, NJ (US);
Yi Zhang, Hillsborough, NJ (US);
Michael Seul, Fanwood, NJ (US);
Nataliya Korzheva, Somerville, NJ (US);
Jiacheng Yang, Hillsborough, NJ (US);
Yi Zhang, Hillsborough, NJ (US);
Bioarray Solutions, Ltd., Warren, NJ (US);
Abstract
A method mediated with in-vitro transcription ('IVT') which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double stranded DNA: wherein, the selected strand forms the template for RNA synthesis. In one embodiment, double stranded DNA incorporating the T7 (or other) promoter sequence at the 3' end or the 5′ end is produced, for example, by amplification of genomic DNA using the Polymerase Chain Reaction (PCR). Also disclosed are nested PCR designs permitting allele analysis in combination with strand selection by IVT. Further, in one embodiment of a homogeneous format for transcription-mediated amplification and multiplexed detection (which may be particularly suited for viral or pathogen detection), encoded microparticles display 'looped' capture probe configurations permitting the generation of a signal upon capture of RNA product and real-time assay monitoring.