The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 14, 2011
Filed:
Oct. 12, 2009
Mark R. Emmett, Monticello, FL (US);
Sasa Kazazic, Zagreb, HR;
Alan G. Marshall, Tallahassee, FL (US);
Michael J. Greig, Carlsbad, CA (US);
Mark R. Emmett, Monticello, FL (US);
Sasa Kazazic, Zagreb, HR;
Alan G. Marshall, Tallahassee, FL (US);
Michael J. Greig, Carlsbad, CA (US);
Florida State University Research Foundation, Tallahassee, FL (US);
Abstract
Analytical methods using hydrogen/deuterium exchange are provided which reduce or eliminate the back-exchange of deuterium for hydrogen. The methods, which are useful in protein and peptide mapping, include the steps of (a) providing a peptide or protein comprising a solvent accessible hydrogen; (b) exchanging the solvent accessible hydrogen for a deuterium; (c) separating the peptide or protein with supercritical fluid chromatography; and (d) analyzing by mass spectrometry the mass of the separated peptide or protein. Supercritical fluid chromatography enables the observation of fast exchanging hydrogen atoms missed using conventional liquid chromatography methods.