The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 14, 2011
Filed:
Jun. 03, 2008
Loren C. Denlinger, Madison, WI (US);
Kirk J. Hogan, Madison, WI (US);
Paul J. Bertics, Oregon, WI (US);
Kathleen Schell, Madison, WI (US);
Loren C. Denlinger, Madison, WI (US);
Kirk J. Hogan, Madison, WI (US);
Paul J. Bertics, Oregon, WI (US);
Kathleen Schell, Madison, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
Abstract
A method of assaying nucleotide receptor P2Xpore activity in white blood cells contained within a mixed cell sample is provided comprising labeling white blood cells with a white blood cell-specific label; depolarizing the labeled white blood cells with an isotonic depolarizing solution; contacting the labeled white blood cells with dye and a P2Xagonist in an amount sufficient to activate P2Xpore activity; contacting the labeled white blood cells with a divalent cation in an amount sufficient to deactivate P2Xpore activity; and analyzing dye uptake whereby P2Xpore activity is quantified by the amount of dye taken up in labeled white blood cells treated with the P2Xagonist relative to labeled white blood cells in the absence of said P2Xagonist, said P2Xpore activity being corrected for sample age and by subtraction of P2Xpore activity contributed by nonviable white blood cells.