The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 07, 2011
Filed:
Sep. 10, 2009
Sergey A. Selifonov, Plymouth, MN (US);
Willem P. C. Stemmer, Los Gatos, CA (US);
Claes Gustafsson, Belmont, CA (US);
Matthew Tobin, San Jose, CA (US);
Stephen Del Cardayre, Belmont, CA (US);
Phillip A. Patten, Menlo Park, CA (US);
Jeremy Minshull, Los Altos, CA (US);
Lorraine J. Giver, Sunnyvale, CA (US);
Sergey A. Selifonov, Plymouth, MN (US);
Willem P. C. Stemmer, Los Gatos, CA (US);
Claes Gustafsson, Belmont, CA (US);
Matthew Tobin, San Jose, CA (US);
Stephen del Cardayre, Belmont, CA (US);
Phillip A. Patten, Menlo Park, CA (US);
Jeremy Minshull, Los Altos, CA (US);
Lorraine J. Giver, Sunnyvale, CA (US);
Codexis Mayflower Holdings LLC, Redwood City, CA (US);
Abstract
In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.