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Abstract

Described is a method for the detection and quantification of methicillin-resistantfrom a specimen comprising a) contacting the specimen with Anti-Protein A antibodies so as to adsorb methicillinresistantand/or methicillin-sensitive, b) separating said antibodies to which methicillin-resistantand/or methicillin-sensitivehave been adsorbed from the specimen, c) lysing methicillin-resistantand/or methicillin-sensitiveadsorbed to said antibodies so as to release their DNA, d) combining the released DNA with (i) probes and/or primers which are specific for a target DNA sequence of the mecA gene of methicillin -resistantand/or of methicillin-resistantepidermis, (ii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistantother than a target DNA sequence of the mecA gene and (iii) probes and/or primers which are specific for a target DNA sequence of methicillin-resistantepidermis other than a target DNA sequence of the mecA gene, whereby the target DNA sequences of methicillin-resistantand of methicillin-resistantepidermis are not homologous, e) subjecting the combined released DNA and the specific probes and/or primers to conditions which permit amplification of said target DNA sequences, f) detecting the presence and amount of the amplified target DNA sequences as an indication of the presence and amount of methicillin-resistant, new primers and probes and diagnostic kits for the detection and quantification of MRSA.