The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 31, 2011
Filed:
Jul. 14, 2008
Albert W. Alsop, Wilmington, DE (US);
Qiong Cheng, Wilmington, DE (US);
Linda Jane Solomon, Wilmington, DE (US);
Stephen R. Fahnestock, Wilmington, DE (US);
Tanja Maria Gruber, Media, PA (US);
Pierre E. Rouviere, Wilmington, DE (US);
Albert W. Alsop, Wilmington, DE (US);
Qiong Cheng, Wilmington, DE (US);
Linda Jane Solomon, Wilmington, DE (US);
Stephen R. Fahnestock, Wilmington, DE (US);
Tanja Maria Gruber, Media, PA (US);
Pierre E. Rouviere, Wilmington, DE (US);
E.I. du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.