The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 31, 2011
Filed:
Apr. 15, 2008
Amy M. Jamruszka-lewis, Sumner, WA (US);
Amy M. Jamruszka-Lewis, Sumner, WA (US);
Weyerhaeuser NR Company, Federal Way, WA (US);
Abstract
In one aspect, the present invention provides methods of producing a synchronized population of conifer somatic embryo germinants in preparation for transplantation. The methods of the invention comprise the steps of: (a) culturing conifer somatic embryos on sterile germination medium for a sufficient period of time to produce germinants comprising a visible, well-defined epicotyl stem of a desired length; (b) selecting a plurality of germinants having an epicotyl stem of the desired length; (c) storing the selected germinants having an epicotyl stem of the desired length produced in step (a) while still on the sterile germination medium from step (a) in a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months; and (d) transferring the germinants stored according to step (c) from the germination medium into water for a time of up to about four weeks at a temperature of about 0.5° C. to about 5° C. prior to planting the selected germinants into growth medium.