The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7939299 B1

PDF
Full Text
Expired
Date of Patent:
May. 10, 2011

Filed:

Aug. 31, 2007
Applicant:

Masafumi Toyama, Yokohama, JP;

Inventor:

Masafumi Toyama, Yokohama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed is a nucleic acid amplification method which is based on a new principle and enables to amplify a nucleic acid having a specific nucleotide sequence in a simple manner, within a short time and with efficiency. The nucleic acid amplification method comprises the steps of: (a) conducting a DNA polymerase chain reaction by using, as a template, DNA comprising a nucleotide sequence to be amplified and using a primer pair having a nucleotide sequence complementary to the nucleotide sequence to be amplified, thereby producing a linear DNA fragment; and (b) conducting a chain-substituting DNA polymerase chain reaction in a chaining manner by using cyclic single-stranded DNA comprising the same nucleotide sequence as that of at least one of the primer pair as a template and employing the 3'-terminus of the linear DNA fragment produced in step (a) as the replication origin.


Find Patent Forward Citations

Toyama, Masafumi. (2011). Nucleic acid amplification method (U.S. Patent No. 7939299). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/7939299

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