The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 2011

Filed:

May. 15, 2009
Applicants:

Atsushi Sugimura, Shiga, JP;

Katsuya Daimon, Kyoto, JP;

Kazutoshi Mihara, Hyogo, JP;

Yae Ito, Hyogo, JP;

Inventors:

Atsushi Sugimura, Shiga, JP;

Katsuya Daimon, Kyoto, JP;

Kazutoshi Mihara, Hyogo, JP;

Yae Ito, Hyogo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/09 (2006.01); A23J 1/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.


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