The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 2011

Filed:

Apr. 05, 2002
Applicants:

Kai Johnsson, Lausanne, CH;

Susanne Gendreizig, Lausane, CH;

Antje Keppler, Lausanne, CH;

Inventors:

Kai Johnsson, Lausanne, CH;

Susanne Gendreizig, Lausane, CH;

Antje Keppler, Lausanne, CH;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/533 (2006.01); C12Q 1/48 (2006.01); C12P 21/00 (2006.01); C12P 21/04 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
Abstract

A method using O-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilisation of the fusion protein.


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