The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 12, 2011
Filed:
Nov. 18, 2005
Barbara Robertson, Boulder, CO (US);
Devin Leake, Denver, CO (US);
Kathryn Robinson, Golden, CO (US);
William S. Marshall, Boulder, CO (US);
Anastasia Khvorova, Boulder, CO (US);
Barbara Robertson, Boulder, CO (US);
Devin Leake, Denver, CO (US);
Kathryn Robinson, Golden, CO (US);
William S. Marshall, Boulder, CO (US);
Anastasia Khvorova, Boulder, CO (US);
Dharmacon, Inc., Lafayette, CO (US);
Abstract
A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least two siRNAs which silences at least a first target gene. The gene silencing composition can be configured such that the at least two siRNAs are each capable of being solubilized or suspended in an aqueous medium in an amount sufficient for transfecting cells in the well. Additionally, the siRNAs can include a hairpin structure, modification, or a conjugate. Also, the at least siRNAs can be rationally designed. The reverse transfection apparatus can be provided as a kit or system that additionally includes cells, polynucleotide carriers, reverse transfection reagents, and the like.