The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 05, 2011
Filed:
Jun. 13, 2007
Hyung-min Chung, Namyangju-si, KR;
Sung-hwan Moon, Seoul, KR;
Ju-mi Kim, Yongin-si, KR;
Soo-hong Lee, Seoul, KR;
Hyung-Min Chung, Namyangju-si, KR;
Sung-Hwan Moon, Seoul, KR;
Ju-Mi Kim, Yongin-si, KR;
Soo-Hong Lee, Seoul, KR;
Chabio & Diostech Co., Ltd., Yongin, KR;
College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation, Pochon-si, KR;
Abstract
The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.