The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2011
Filed:
Dec. 08, 2006
Robert Haselbeck, San Diego, CA (US);
Mark Hilgers, San Diego, CA (US);
Karen Shaw, Poway, CA (US);
Vickie Brown-driver, Solana Beach, CA (US);
Kedar Gc, San Diego, CA (US);
John M. Finn, Encinitas, CA (US);
Mark Stidham, San Diego, CA (US);
Robert Haselbeck, San Diego, CA (US);
Mark Hilgers, San Diego, CA (US);
Karen Shaw, Poway, CA (US);
Vickie Brown-Driver, Solana Beach, CA (US);
Kedar Gc, San Diego, CA (US);
John M. Finn, Encinitas, CA (US);
Mark Stidham, San Diego, CA (US);
Trius Therapeutics, Inc., San Diego, CA (US);
Abstract
The invention provides a method for generating and selecting drug-sensitizing antisense DNA fragments. In one embodiment, the method includes identifying a gene of interest using knowledge of bacterial physiology, biochemistry, genetics, genomics, and other means. The method includes PCR amplification of a gene of interest using genomic DNA as a template; fragmentation of the DNA by sonication or other means; selecting DNA fragments no longer than 400 base pairs; ligating the DNA fragments into a suitable expression plasmid with a selectable marker; transforming the plasmids containing the DNA fragments into the organism from which the gene of interest originated; and selecting clones from transformed cells that show a phenotypic difference of the clone grown in the presence of the inducer relative to the phenotype in the absence of inducer.