The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2011
Filed:
Dec. 04, 2004
Ji Sook Park, Seoul, KR;
Jong Sang Chung, Seoul, KR;
Min Ji Baek, Yongin, KR;
Jee Won Ahn, Seoul, KR;
Ki Wan Kim, Seoul, KR;
Hyung Ki Park, Seoul, KR;
Dong Eok Lee, Seoul, KR;
Myung Suk OH, Suwon, KR;
Ji Sook Park, Seoul, KR;
Jong Sang Chung, Seoul, KR;
Min Ji Baek, Yongin, KR;
Jee Won Ahn, Seoul, KR;
Ki Wan Kim, Seoul, KR;
Hyung Ki Park, Seoul, KR;
Dong Eok Lee, Seoul, KR;
Myung Suk Oh, Suwon, KR;
CJ Cheiljedang Corporation, Seoul, KR;
Abstract
Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and cation exchange chromatography, wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.