The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2011
Filed:
Oct. 22, 2008
Robert Charles Ladner, Ijamsville, MD (US);
Sonia Kosow Guterman, Belmont, MA (US);
Bruce Lindsay Roberts, Southborough, MA (US);
William Markland, Southborough, MA (US);
Arthur Charles Ley, Newton, MA (US);
Rachel Baribault Kent, Boxborough, MA (US);
Robert Charles Ladner, Ijamsville, MD (US);
Sonia Kosow Guterman, Belmont, MA (US);
Bruce Lindsay Roberts, Southborough, MA (US);
William Markland, Southborough, MA (US);
Arthur Charles Ley, Newton, MA (US);
Rachel Baribault Kent, Boxborough, MA (US);
Dyax Corp., Cambridge, MA (US);
Abstract
In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore or phage (genetic package) are introduced into a genetic package. The protein is expressed and the potential binding domain is displayed on the outer surface of the package. The cells or viruses bearing the binding domains which recognize the target molecule are isolated and amplified. The successful binding domains are then characterized. One or more of these successful binding domains is used as a model for the design of a new family of potential binding domains, and the process is repeated until a novel binding domain having a desired affinity for the target molecule is obtained. In one embodiment, the first family of potential binding domains is related to bovine pancreatic trypsin inhibitor, the genetic package is M13 phage, and the protein includes the outer surface transport signal of the M13 gene III protein.