The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2011
Filed:
Aug. 02, 2004
David M. Neville, Bethesda, MD (US);
Jung-hee Woo, Rockville, MD (US);
Yuan-yi Liu, Potomac, MD (US);
David M. Neville, Bethesda, MD (US);
Jung-Hee Woo, Rockville, MD (US);
Yuan-Yi Liu, Potomac, MD (US);
Abstract
The present invention relates to a method of expressing an immunotoxin instrain mutated to toxin resistance comprising a) growing thein a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.