Method for producing a compound of interest in a filamentous fungal cell

Abstract

The invention relates to a nucleotide sequence comprising; a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, the synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally the nucleotide sequence comprises control sequences such as; one translational termination sequence orientated in 5' towards 3′ direction selected from the following list of sequences; TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5′ towards 3′ direction selected from the following list of sequences; gctnccyyc (SEQ ID NO:20), using ambiguity codes for nucleotides; v (A/C/G); n (A/C/G/T), preferably 5′-GCT TCC TTC-3′ (SEQ ID NO:21). The invention further relates to a consensus translational initiator sequence; 5′-mwChkyCAmv-3′ (SEQ ID NO:16), preferably the translational initiator sequence is selected from the list consisting of; 5′-mwChkyCAAA-3′ (SEQ ID NO:17), 5′-mwChkyCACA-3′ (SEQ ID NO:18), and 5′-mwChkyCAAG-3′ (SEQ ID NO:19).