The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 15, 2011
Filed:
Mar. 01, 2005
Jan Barnikow, Basel, CH;
Christopher Chidley, Crissier, CH;
Thomas Gronemeyer, Hattingen, DE;
Christian Heinis, Aarberg, CH;
Hughes Jaccard, Yverdon-les-Bains, CH;
Kai Johnsson, Lausanne, CH;
Alexandre Juillerat, Paris, FR;
Antje Keppler, Heidelberg, DE;
Jan Barnikow, Basel, CH;
Christopher Chidley, Crissier, CH;
Thomas Gronemeyer, Hattingen, DE;
Christian Heinis, Aarberg, CH;
Hughes Jaccard, Yverdon-les-Bains, CH;
Kai Johnsson, Lausanne, CH;
Alexandre Juillerat, Paris, FR;
Antje Keppler, Heidelberg, DE;
Ecole Polytechnique Federale de Lausanne, Lausanne, CH;
Abstract
The invention relates to AGT mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction; (b) localisation of the expressed protein in eukaryotic cells that is no longer restricted to the nucleus; (c) improved expression yield as soluble protein and improved stability in various hosts; (d) improved stability under oxidising conditions; (e) improved stability within cells after reaction with a substrate; (f) improved stability outside cells before and after reaction with a substrate; (g) improved in vitro solubility; (h) improved reactivity against O-alkylguanine substrates; (1) reduced reactivity against DNA-based substrates; and (j) reduced reactivity against N-substituted O-alkylguanine substrates. Such AGT mutants with the mentioned improved properties are mutants wherein between 1 and 25 amino acids of the wild type human AGT are substituted by other amino acids, and optionally 1 to 5 amino acids out of the continuous chain at one, two or three positions are deleted or added and/or 1 to 4 amino acids at the N-terminus or 1 to 40 amino acids at the C-terminus are deleted. The invention further relates to a method for detecting and/or manipulating a protein of interest wherein the protein of interest is incorporated into a fusion protein with the AGT mutants of the invention. Another object of the invention are AGT fusion proteins comprising such AGT mutants and the protein of interest.