The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 04, 2011
Filed:
Jan. 22, 2009
Michael J. Domanico, Longmont, CO (US);
Matt Myers, Boulder, CO (US);
Kristian Keane, Boulder, CO (US);
Lisa R. Braun, Denver, CO (US);
Thomas Kolzau, Hamburg, DE;
Michael J. Domanico, Longmont, CO (US);
Matt Myers, Boulder, CO (US);
Kristian Keane, Boulder, CO (US);
Lisa R. Braun, Denver, CO (US);
Thomas Kolzau, Hamburg, DE;
Qiagen North American Holdings, Inc., Valencia, CA (US);
Abstract
Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1 μm.