The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 28, 2010
Filed:
Nov. 08, 2005
Gerd Gellissen, Wülfrath, DE;
Jens Klabunde, Düsseldorf, DE;
Cornelis Hollenberg, Düsseldorf, DE;
Adelheid Degelmann, Düsseldorf, DE;
Gerd Gellissen, Wülfrath, DE;
Jens Klabunde, Düsseldorf, DE;
Cornelis Hollenberg, Düsseldorf, DE;
Adelheid Degelmann, Düsseldorf, DE;
Artes Biotechnology GmbH, Essen, DE;
Abstract
A heterologous protein is produced by a method in which yeast-type host cells are prepared, which, in addition to the at least one endogenous homologous DNA sequence coding for a first calnexin, includes at least one recombinant DNA sequence coding for the protein and at least one additional recombinant DNA sequence coding for at least one second calnexin. The protein in the host cell represents a heterologous secretable protein. The host cells are induced to express the heterologous protein via the at least one sequence coding for the protein and to overexpress calnexins by the at least one additional sequence coding for the at least one second calnexin. The host cells are then induced to secrete the heterologous protein. The secreted heterologous protein is then separated off.