The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 21, 2010

Filed:

Sep. 28, 2006
Applicants:

Avi J. Ashkenazi, San Mateo, CA (US);

Sarah Hymowitz, San Francisco, CA (US);

Robert F. Kelley, San Bruno, CA (US);

Iphigenia Koumenis, Winston-Salem, NC (US);

Woon-lam Susan Leung, San Mateo, CA (US);

Mark O'connell, Montara, CA (US);

Roger Pai, Los Altos, CA (US);

Zahra Shahrokh, Weston, MA (US);

Laura Simmons, Burlingame, CA (US);

Inventors:

Avi J. Ashkenazi, San Mateo, CA (US);

Sarah Hymowitz, San Francisco, CA (US);

Robert F. Kelley, San Bruno, CA (US);

Iphigenia Koumenis, Winston-Salem, NC (US);

Woon-Lam Susan Leung, San Mateo, CA (US);

Mark O'Connell, Montara, CA (US);

Roger Pai, Los Altos, CA (US);

Zahra Shahrokh, Weston, MA (US);

Laura Simmons, Burlingame, CA (US);

Assignee:

Genentech, Inc., South San Francisco, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/10 (2006.01); C12N 15/12 (2006.01); C07K 14/52 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.


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