The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2010
Filed:
Aug. 17, 2006
Alejandro Eduardo Maass Sepúlveda, Santiago, CL;
Andrés Octavio Aravena Duarte, Santiago, CL;
Mauricio Alejandro Gonzalez Canales, Santiago, CL;
Servet Martinez Aguilera, Santiago, CL;
Pilar Angélica Parada Valdecantos, Santiago, CL;
Katia Nicole Ehrenfeld Stolzenbach, Santiago, CL;
Alejandro Eduardo Maass Sepúlveda, Santiago, CL;
Andrés Octavio Aravena Duarte, Santiago, CL;
Mauricio Alejandro Gonzalez Canales, Santiago, CL;
Servet Martinez Aguilera, Santiago, CL;
Pilar Angélica Parada Valdecantos, Santiago, CL;
Katia Nicole Ehrenfeld Stolzenbach, Santiago, CL;
Biosigma S.A., Colina, CL;
Abstract
A method of designing oligonucleotides useful in molecular biology techniques as PCR primers or in other techniques as identification and/or quantification probes is disclosed. The method permits designing specific oligonucleotides for the identification of a determined sequence in a metagenomic sample. The method includes selecting or constructing a database of reference sequences, selecting a subset of sequences belonging to target organisms, selecting candidate oligonucleotides from such sequences, depurating these candidate oligonucleotides according to hybridization specificity and thermodynamic stability criteria, obtaining a list of designed oligonucleotides that fulfill the hybridization specificity and thermodynamic stability criteria, producing materially by chemical synthesis the designed oligonucleotides, and selecting the oligonucleotides which comply with the requirements of the desired process.