The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 14, 2010
Filed:
Jan. 08, 2004
David H. Reifsnyder, El Cerrito, CA (US);
Duane Inlow, Alamo, CA (US);
Glenn Dorin, San Rafael, CA (US);
Patricio T. Riquelme, Walnut Creek, CA (US);
Cynthia A. Cowgill, Berkeley, CA (US);
Douglas G. Bolesch, Berkeley, CA (US);
Mark E. Gustafson, St. Charles, MO (US);
David H. Reifsnyder, El Cerrito, CA (US);
Duane Inlow, Alamo, CA (US);
Glenn Dorin, San Rafael, CA (US);
Patricio T. Riquelme, Walnut Creek, CA (US);
Cynthia A. Cowgill, Berkeley, CA (US);
Douglas G. Bolesch, Berkeley, CA (US);
Mark E. Gustafson, St. Charles, MO (US);
Novartis Vaccines and Diagnostics, Inc., Emeryville, CA (US);
Pharmacia & Upjohn Company, Kalamazoo, MI (US);
Abstract
Highly purified preparations of TFPI or TFPI analogs can be prepared using a method that generally involves the following steps: (1) expression of TFPI or TFPI analog in, (2) isolation of refractile bodies, (3) dissolution of the refractile bodies and refolding of the expressed TFPI or TFPI analog, (4) SP-Sepharose fast flow (FF) chromatography, (5) a first concentration and diafiltration step, (6) Q-Sepharose high (HP) performance chromatography, (7) butyl hydrophobic interaction chromatography (HIC), (8) SP-Sepharose HP chromatography, and (9) a second concentration/diafiltration step. Less than about 12% of the TFPI or TFPI analog molecules in such preparations are modified TFPI or TFPI analog species (i.e., oxidized, carbamylated, acetylated, deamidated, aggregated, or misfolded species).