The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 30, 2010
Filed:
Nov. 08, 2004
Richard B. Thompson, Baltimore, MD (US);
Daniel Elbaum, Newton, MA (US);
Vincent L. Feliccia, Arnold, MD (US);
David Christianson, Media, PA (US);
Marcia W. Patchan, Columbia, MD (US);
Zhengfang GE, Rockville, MD (US);
Badri P. Maliwal, San Francisco, CA (US);
Richard B. Thompson, Baltimore, MD (US);
Daniel Elbaum, Newton, MA (US);
Vincent L. Feliccia, Arnold, MD (US);
David Christianson, Media, PA (US);
Marcia W. Patchan, Columbia, MD (US);
Zhengfang Ge, Rockville, MD (US);
Badri P. Maliwal, San Francisco, CA (US);
Other;
Abstract
The invention relates to compositions and kits for homogeneous fluorescence polarization (anisotropy) assays for detecting and quantifying metal ions in solution. Metal-dependent binding of a fluorescent ligand to an unlabeled macromolecule effects a measurable change in anisotropy as will the binding of metal ions to a fluorescent labeled macromolecule. Binding of the fluorescent ligand to the unlabeled macromolecule is metal dependent with the change in anisotropy being proportional to the concentration of bound metal ions. Conversely, if the fluorescent label is first conjugated to a macromolecule and the macromolecule is subsequently stripped of metal ion, it may then be used to signal binding of metal ions. The covalently bound fluorescent label exhibits changes in anisotropy proportional to the concentration of bound metal ions. Kits comprise a fluorescent molecule and a macromolecule.