The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 26, 2010
Filed:
Feb. 29, 2008
Michael Baldwin, Milwaukee, WI (US);
Marite Bradshaw, Madison, WI (US);
William H. Tepp, Stoughton, WI (US);
Eric A. Johnson, Madison, WI (US);
Joseph T. Barbieri, Milwaukee, WI (US);
Christina L. Pier, Fitchburg, WI (US);
Michael Baldwin, Milwaukee, WI (US);
Marite Bradshaw, Madison, WI (US);
William H. Tepp, Stoughton, WI (US);
Eric A. Johnson, Madison, WI (US);
Joseph T. Barbieri, Milwaukee, WI (US);
Christina L. Pier, Fitchburg, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
Abstract
A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparingtransformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.