Quinazoline-containing kits for labeling aldehyde or ketone moieties

Abstract

Kits including novel fluorescent derivitization reagents for labeling an aldehyde- and/or ketone-containing target substance in a sample are described. In one embodiment kits are provided the novel fluorescent derivitization reagents of which have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and Ris -L-Z; L is arylene, or a Cperfluoroalkylene; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R-Rare independently H, Calkyl, Calkylamino, amino, or halogen; and R-Rare independently H, Calkyl, Calkoxy, Cperfluoroalkyl, Calkylamino, di(C-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The kits and reagents described are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.