The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 2010
Filed:
Feb. 01, 2010
YE Fang, Painted Post, NY (US);
Ann M. Ferrie, Painted Post, NY (US);
Yulong Hong, Painted Post, NY (US);
Sadashiva K. Pai, Painted Post, NY (US);
Jinlin Peng, Painted Post, NY (US);
Brian L. Webb, Painted Post, NY (US);
Ye Fang, Painted Post, NY (US);
Ann M. Ferrie, Painted Post, NY (US);
Yulong Hong, Painted Post, NY (US);
Sadashiva K. Pai, Painted Post, NY (US);
Jinlin Peng, Painted Post, NY (US);
Brian L. Webb, Painted Post, NY (US);
Corning Incorporated, Corning, NY (US);
Abstract
Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5'-diphosphate (GDP) salt, and/or an anti-oxidant reagent.