The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 19, 2010
Filed:
Jul. 16, 2009
Roland Carlsson, Lund, SE;
Ann-christin Malmborg Hager, Helsingborg, SE;
Christina Furebring, Lund, SE;
Carl Borrebaeck, Hjarup, SE;
Roland Carlsson, Lund, SE;
Ann-Christin Malmborg Hager, Helsingborg, SE;
Christina Furebring, Lund, SE;
Carl Borrebaeck, Hjarup, SE;
Alligator Bioscience AB, Lund, SE;
Abstract
The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of a) providing single stranded DNA constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide sequences with a nuclease other than DNase I to generate populations of single stranded fragments; c) contacting the fragments generated from the plus strands with fragments generated from the minus strands and optionally, adding primer sequences that anneal to the 3' and 5′ ends of at least one of the parent polynucleotides under annealing conditions; and d) amplifying the fragments that anneal to each other to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics as compared to the one or more protein motifs encoded by the parent polynucleotides.