The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 12, 2010
Filed:
May. 17, 2006
Arundhati Mandal, Navi Mumbai, IN;
Shabari Tipnis, Navi Mumbai, IN;
Geeta Ravindran, Navi Mumbai, IN;
Jayant Kulkarni, Navi Mumbai, IN;
Ameet Patki, Mumbai, IN;
Rajarshi Pal, Navi Mumbai, IN;
Bipasha Bose, Navi Mumbai, IN;
Alam Firdos Kahn, Navi Mumbai, IN;
Aparna Khanna, Navi Mumbai, IN;
Arundhati Mandal, Navi Mumbai, IN;
Shabari Tipnis, Navi Mumbai, IN;
Geeta Ravindran, Navi Mumbai, IN;
Jayant Kulkarni, Navi Mumbai, IN;
Ameet Patki, Mumbai, IN;
Rajarshi Pal, Navi Mumbai, IN;
Bipasha Bose, Navi Mumbai, IN;
Alam Firdos Kahn, Navi Mumbai, IN;
Aparna Khanna, Navi Mumbai, IN;
Abstract
Purified preparations of human embryonic stem cells with certain population-specific characteristics are disclosed. This preparation is characterized by the positive expression of the following pluripotent cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); alkaline phosphatase (+), as well as a set of ES cell markers including Oct-4, Nanog, Rex1, Sox2, Thy1, FGF4, ABCG2, Dppa5, UTF1, Cripto1, hTERT, Connexin-43 and Connexin-45. The cells of the preparation are negative for lineage specific markers like Keratin 8, Sox-1, NFH (ectoderm), MyoD, brachyury, cardiac-actin (mesoderm), HNF-3 beta, albumin, and PDX1 (endoderm). The cells of the preparation are human embryonic stem cells, have normal karyotypes, exhibit high telomerase activity and continue to proliferate in an undifferentiated state after continuous culture for over 40 passages. The embryonic stem cell line Relicell™ hES1 also retains the ability, throughout the culture, to differentiate into cell and tissue types derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). Methods for isolating a human embryonic stem cell line are also disclosed.