The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2010
Filed:
Jan. 24, 2006
Stephen Van Dien, Kanagawa, JP;
Shintaro Iwatani, Kanagawa, JP;
Yoshihiro Usuda, Kanagawa, JP;
Kazuhiko Matsui, Kanagawa, JP;
Takuji Ueda, Kanagawa, JP;
Yuichiro Tsuji, Kanagawa, JP;
Stephen Van Dien, Kanagawa, JP;
Shintaro Iwatani, Kanagawa, JP;
Yoshihiro Usuda, Kanagawa, JP;
Kazuhiko Matsui, Kanagawa, JP;
Takuji Ueda, Kanagawa, JP;
Yuichiro Tsuji, Kanagawa, JP;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.