The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 2010
Filed:
Apr. 02, 2008
Christopher L. Baszczynski, Earlham, IA (US);
Lesek Alexander Lyznik, Johnston, IA (US);
William J. Gordon-kamm, Urbandale, IA (US);
Xueni Guan, San Diego, CA (US);
Christopher L. Baszczynski, Earlham, IA (US);
Lesek Alexander Lyznik, Johnston, IA (US);
William J. Gordon-Kamm, Urbandale, IA (US);
Xueni Guan, San Diego, CA (US);
Pioneer Hi-Bred International, Inc., Johnston, IA (US);
Abstract
The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.