The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 28, 2010
Filed:
Oct. 29, 2003
Yasumasa Mitani, Hiroshima-Ken, JP;
Akio Yamane, Hiroshima-Ken, JP;
Yuko Shibata, Kanagawa, JP;
Yoshihide Hayashizaki, Ibaraki-Ken, JP;
Yasumasa Mitani, Hiroshima-Ken, JP;
Akio Yamane, Hiroshima-Ken, JP;
Yuko Shibata, Kanagawa, JP;
Yoshihide Hayashizaki, Ibaraki-Ken, JP;
Riken, Saitama, JP;
Kabushiki Kaisha Dnaform, Kanagawa, JP;
Abstract
The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3'-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the target nucleic acid sequence, and in the 5′-side of the sequence (Ac′) a sequence (B′) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5′-side of the sequence (A) on the target nucleic acid sequence, wherein {X−(Y−Y′)}/X is in the range of −1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac′), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y′ denotes the number of bases in an intervening sequence between the sequences (Ac′) and (B′) (Y′ may be zero).