The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 21, 2010
Filed:
May. 25, 2005
Richard A. Mathies, Moraga, CA (US);
Robert Blazej, Berkeley, CA (US);
Chung Liu, Albany, CA (US);
Palani Kumaresan, Berkeley, CA (US);
Stephanie H. I. Yeung, Berkeley, CA (US);
Richard A. Mathies, Moraga, CA (US);
Robert Blazej, Berkeley, CA (US);
Chung Liu, Albany, CA (US);
Palani Kumaresan, Berkeley, CA (US);
Stephanie H. I. Yeung, Berkeley, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA.