The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 14, 2010
Filed:
Jan. 31, 2007
Applicants:
Nathalie Scholler, Seattle, WA (US);
Barbara Garvik, Seattle, WA (US);
Inventors:
Nathalie Scholler, Seattle, WA (US);
Barbara Garvik, Seattle, WA (US);
Assignee:
Fred Hutchinson Cancer Research Center, Seattle, WA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12P 21/06 (2006.01); C12P 9/00 (2006.01);
U.S. Cl.
CPC ...
Abstract
The present invention provides compositions and methods for generating in vivo biotinylated, secreted recombinant polypeptides comprising recombinantly co-expressing in a yeast cell (a) a secreted recombinant polypeptide comprising a biotin accepting site; and (b) a biotin ligase fused to the golgi localization domain so that the biotin ligase is localized to the golgi of the yeast cell where it acts on the biotin-accepting site of the secreted polypeptide as it transits the secretory system.