The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 14, 2010
Filed:
Nov. 03, 2008
Qiong Cheng, Wilmington, DE (US);
Kevin Michael Croker, Hockessin, DE (US);
Stephen R. Fahnestock, Wilmington, DE (US);
Tanja Maria Gruber, Media, PA (US);
Kristin Ruebling-jass, Wilmington, DE (US);
Jianzhong Zhang, Wilmington, DE (US);
Qiong Cheng, Wilmington, DE (US);
Kevin Michael Croker, Hockessin, DE (US);
Stephen R. Fahnestock, Wilmington, DE (US);
Tanja Maria Gruber, Media, PA (US);
Kristin Ruebling-Jass, Wilmington, DE (US);
Jianzhong Zhang, Wilmington, DE (US);
E.I. du Pont de Nemours and Company, Wilmington, DE (US);
Abstract
A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.