The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 31, 2010
Filed:
Feb. 12, 2007
Stephen P. Eisenberg, Boulder, CO (US);
Casey C. Case, San Mateo, CA (US);
George N. Cox, Iii, Louisville, CO (US);
Andrew Jamieson, San Francisco, CA (US);
Edward J. Rebar, Berkeley, CA (US);
Stephen P. Eisenberg, Boulder, CO (US);
Casey C. Case, San Mateo, CA (US);
George N. Cox, III, Louisville, CO (US);
Andrew Jamieson, San Francisco, CA (US);
Edward J. Rebar, Berkeley, CA (US);
Sangamo Biosciences, Inc., Richmond, CA (US);
Abstract
The invention provides criteria and methods for selecting optimum subsequence(s) from a target gene for targeting by a zinc finger protein. Some of the methods of target site selection seek to identify one or more target segments having a DNA motif containing one or more so-called D-able subsites having the sequence 5'NNGK3′. Other methods of the invention are directed to selection of target segments within target genes using a correspondence regime between different triplets of three bases and the three possible positions of a triplet within a nine-base site. In another aspect, the invention provides methods of designing zinc finger proteins that bind to a preselected target site. These methods can be used following the preselection of target sites according to the procedures and criteria described above. The methods of design use a database containing information about previously characterized zinc finger proteins.