The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 17, 2010

Filed:

Mar. 14, 2008
Applicants:

Mitsuyoshi Okuda, Haga-gun, JP;

Tsuyoshi Sato, Haga-gun, JP;

Kazuhiro Saito, Haga-gun, JP;

Nobuyuki Sumitomo, Haga-gun, JP;

Yoshifumi Izawa, Haga-gun, JP;

Katsuhisa Saeki, Haga-gun, JP;

Tohru Kobayashi, Haga-gun, JP;

Masafumi Nomura, Wakayama, JP;

Inventors:

Mitsuyoshi Okuda, Haga-gun, JP;

Tsuyoshi Sato, Haga-gun, JP;

Kazuhiro Saito, Haga-gun, JP;

Nobuyuki Sumitomo, Haga-gun, JP;

Yoshifumi Izawa, Haga-gun, JP;

Katsuhisa Saeki, Haga-gun, JP;

Tohru Kobayashi, Haga-gun, JP;

Masafumi Nomura, Wakayama, JP;

Assignee:

Kao Corporation, Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/50 (2006.01); C11D 3/386 (2006.01); C12N 9/54 (2006.01); C12N 15/74 (2006.01);
U.S. Cl.
CPC ...
Abstract

Provided in the present invention is an alkaline protease wherein an amino acid residue at (a) position 65, (b) position 101, (c) position 163, (d) position 170, (e) position 171, (f) position 273, (g) position 320, (h) position 359 or (i) position 387 of SEQ. ID NO:1 or at a position corresponding thereto has been selected from the following amino acid residues: position (a): proline, position (b): asparagine, position (c): histidine, aspartic acid, phenylalanine, lysine, asparagine, serine, isoleucine, leucine, glutamine, threonine and valine, position (d): valine and leucine, position (e): alanine, glutamic acid, glycine and threonine, position (f): isoleucine, glycine and threonine, position (g): phenylalanine, valine, threonine, leucine, isoleucine and glycine, position (h): serine, leucine, valine, isoleucine and glutamine, position (i): alanine, lysine, glutamine, glutamic acid, arginine and histidine. The present invention makes it possible to efficiently produce and provide alkaline proteases having activity even in the presence of a highly concentrated fatty acid, and exhibiting excellent detergency for the removal of a complex stain containing protein, sebum and the like, and therefore being useful as an enzyme to be incorporated in a detergent.


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