The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 27, 2010
Filed:
May. 01, 2007
David A. Haake, Culver City, CA (US);
Bernard M. Churchill, Pacific Palisades, CA (US);
Joseph C. Liao, Los Altos, CA (US);
Marc A. Suchard, Los Angeles, CA (US);
Yang LI, Northridge, CA (US);
Mitra Mastali, Marina Del Rey, CA (US);
David A. Haake, Culver City, CA (US);
Bernard M. Churchill, Pacific Palisades, CA (US);
Joseph C. Liao, Los Altos, CA (US);
Marc A. Suchard, Los Angeles, CA (US);
Yang Li, Northridge, CA (US);
Mitra Mastali, Marina Del Rey, CA (US);
The Regents of the University of California, Oakland, CA (US);
The United States of America as represented by the Department of Veterans Affairs, Washington, DC (US);
Abstract
Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.