The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 13, 2010
Filed:
Mar. 02, 2005
Johannes Auer, Schwaigen, DE;
Apollon Papadimitriou, Bichl, DE;
Christian Schantz, Munich, DE;
Stefan Seeber, Penzberg, DE;
Johannes Auer, Schwaigen, DE;
Apollon Papadimitriou, Bichl, DE;
Christian Schantz, Munich, DE;
Stefan Seeber, Penzberg, DE;
Hoffman-La Roche Inc., Nutley, NJ (US);
Abstract
A method for the production of the α-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.