The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 13, 2010
Filed:
Sep. 02, 2004
David John Glover, West Drayton, GB;
Mukesh Sehdev, Slough, GB;
Dominic Gambier Reeks, High Wycombe, GB;
David John Glover, West Drayton, GB;
Mukesh Sehdev, Slough, GB;
Dominic Gambier Reeks, High Wycombe, GB;
UCB Pharma S.A., Brussels, BE;
Abstract
The present invention provides a method for controlling the partitioning of a recombinant protein between the supernatant and the periplasm inhost cell cultures wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing anhost cell culture b) changing the growth rate of thehost cells c) inducing expression of the recombinant protein wherein steps (b) and (c) can be performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the culture supernatant and thehost cell periplasm e) comparing the yield determined in step (d) with the yield determined when at least one other growth rate has been used in step (b) f) selecting a growth rate from the comparison made in step (e) in which the partitioning of the recombinant protein between the supernatant and the periplasm is most suited to the primary recovery of the recombinant protein.