The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 06, 2010
Filed:
Sep. 17, 2004
Martin B. Oleksiewicz, Bronshoj, DK;
Lars S. Nielsen, Niva, DK;
Peter S. Andersen, Vanlose, DK;
Margit H. Hansen, Copenhagen, DK;
Martin B. Oleksiewicz, Bronshoj, DK;
Lars S. Nielsen, Niva, DK;
Peter S. Andersen, Vanlose, DK;
Margit H. Hansen, Copenhagen, DK;
Symphogen A/S, Lyngby, DK;
Abstract
Multiplex overlap-extension RT-PCR provides an efficient method of linking two or more nucleotide sequences encoding for domains or subunits of a heteromeric protein, in a single reaction. Especially, the linkage of variable region encoding sequences from e.g. immunoglobulins, T cell receptors or B cell receptors is eased with the method of the present invention. This allows for a more efficient way of generating libraries of variable region encoding sequences. The capability to perform the multiplex overlap-extension RT-PCR using template derived from an isolated single cell enables the generation of cognate pair libraries in a high-throughput format.