Bacteria sensor and method

Abstract

Bacteria accumulations on the interior walls of a fluid conduit are detected by placing a bacterial target substrate in the conduit. The substrate is structured to allow bacteria to colonize it at at least the rate of accumulation expected on the conduit walls or at an accelerated rate in order to preempt normal bacteria accumulation on the walls. A bacteria getter may be used to accelerate bacterial colonization of the substrate. An excitation signal interrogating the substrate causes autofluorescence in the presence of bacteria, specifically from NADH and/or NADPH present. The autofluorescent emission is transmitted to a detector and processor. In one system when the presence of bacteria at a preset level is detected there is initiated a diversion of the fluid into an auxiliary subsystem during which the primary subsystem is remediated. In one configuration, a wall portion is transparent and the biofilm target substrate is integral with the transparent wall portion and the sensor head is attached to the outside of the transparent wall portion. It can be made as a removable and/or disposable cell in which the transparent wall portion is a glass plug that fits into a hole in the conduit. The biofilm target substrate can be a getter affinity surface formed on the inside surface of the glass plug. Various means are used for obtaining accelerated biofilm growth.