The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7736643 B1

PDF
Full Text
Expired
Date of Patent:
Jun. 15, 2010

Filed:

Sep. 04, 2003
Applicants:

Eui-sung Choi, Taejeon-si, KR;

Jung-hoon Sohn, Taejeon-si, KR;

So-young Kim, Gwacheon-si, KR;

Inventors:

Eui-Sung Choi, Taejeon-si, KR;

Jung-Hoon Sohn, Taejeon-si, KR;

So-Young Kim, Gwacheon-si, KR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/46 (2006.01); C12N 9/20 (2006.01); C12N 15/63 (2006.01); C12N 1/15 (2006.01); C07K 14/00 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry.


Find Patent Forward Citations

Choi, Eui-Sung. (2010). Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase (U.S. Patent No. 7736643). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/7736643

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