The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 08, 2010
Filed:
Oct. 13, 2008
Mara Boenitz-dulat, Tutzing, DE;
Daniela Beck, Penzberg, DE;
Peter Kratzsch, Penzberg, DE;
Rainer Schmuck, Benediktbeuern, DE;
Herbert Von Der Eltz, Weilheim, DE;
Mara Boenitz-Dulat, Tutzing, DE;
Daniela Beck, Penzberg, DE;
Peter Kratzsch, Penzberg, DE;
Rainer Schmuck, Benediktbeuern, DE;
Herbert Von Der Eltz, Weilheim, DE;
Roche Diagnostics Operations, Inc., Indianapolis, IN (US);
Abstract
A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from thes-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.