The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 01, 2010
Filed:
Jan. 26, 2009
Matthew P. Collis, Thomasville, PA (US);
Donald W. Copertino, Catonsville, MD (US);
Karen Eckert, Perry Hall, MD (US);
Thomas L. Fort, Finksburg, MD (US);
Matthew P. Collis, Thomasville, PA (US);
Donald W. Copertino, Catonsville, MD (US);
Karen Eckert, Perry Hall, MD (US);
Thomas L. Fort, Finksburg, MD (US);
Becton Dickinson and Company, Franklin Lakes, NJ (US);
Abstract
The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.