The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 25, 2010
Filed:
Jun. 26, 2007
Suk-tae Kwon, Suwon-si, KR;
Mi-sun Lee, Suwon-si, KR;
Gun-a Kim, Anyang-si, KR;
Jung-hyun Lee, Seongnam-si, KR;
Suk-Tae Kwon, Suwon-si, KR;
Mi-Sun Lee, Suwon-si, KR;
Gun-A Kim, Anyang-si, KR;
Jung-Hyun Lee, Seongnam-si, KR;
Sungkyunkwan University Foundation for Corporate Collaboration, Suwon, Gyeonggi-Do, KR;
Abstract
The present invention provides uracil-DNA glycosylase (UDG) gene originating fromsp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.